The same three-step transcriptomic analysis strategy was applied to each separate study: (i) data acquisition and preprocessing, (ii) differential gene expression and functional enrichment analysis, and (iii) differential pathway activation and functional analysis. The functional results of all studies were integrated using meta-analytical techniques (Fig. (Fig.1).1). Bioinformatics analysis was carried out using the programming language R 3.6.0 [12]; information regarding the packages used and their version numbers are provided in the supplementary material.
Data analysis workflow
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