Dual luciferase assay

WC Wen-Teng Chang
MH Ming-Yuan Hong
CC Chien-Liang Chen
CH Chi-Yuan Hwang
CT Cheng-Chieh Tsai
CC Chia-Chang Chuang
request Request a Protocol
ask Ask a question
Favorite

After 24 h of LPS (1 μg/mL) or DEX (10 μM/mL) treatment, the cells were washed with PBS, and then, lysates were prepared by scraping the cells from plates in the presence of 1× passive lysis buffer (Promega). The luciferase assay was performed using the dual luciferase assay system (Promega) and then detected using the Sirius luminometer (Berthold Detection System, Pforzheim, Germany).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A