DSS-Induced Acute UC in Mice and (±)-8-ADC Treatment

C57BL/6J mice were randomly divided into 7 groups of 6 mice each: control (normal water), vehicle (300 mg/kg (±)-8-ADC), DSS-induced (2.3% DSS in drinking water), SASP (300 mg/kg), and three (±)-8-ADC (75, 150, and 300 mg/kg) groups. The vehicle group was received 300 mg/kg (±)-8-ADC but was not treated with DSS. Except for the control and the vehicle groups, colitis was induced in all mice by orally administering 2.3% DSS for 7 consecutive days. The SASP group was treated with 300 mg/kg SASP, and the (±)-8-ADC groups received 75, 150, or 300 mg/kg (±)-8-ADC orally from the start until the end of the experiment. The DAI score was determined over the course of 7 days as previously described (Kaser et al., 2008), and was as follows: weight loss (%) (0: normal, 1:1–5%, 2:5–10%, 3:10–15%, and 4:>15%), stool consistency (0 and 1: normal, 2 and 3: loose stool, and 4: diarrhea), and stool blood (0: negative, 1:±, 2:+, 3:++, and 4: gross). After the 7-day experimental period, the mice were sacrificed. The fecal occult blood test was performed as described by the manufacturer’s instructions. The colons of the mice were excised, opened longitudinally, and rinsed with an ice-cold saline solution. The excised colons were then divided longitudinally into two parts, where one part was immediately fixed in 10% formalin for histological assays and the other was immediately frozen at -80°C for the MPO and cytokine assays.