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Tumor xenograft

LM Ling Mao
XW Xiaoweng Wu
ZG Zhengpeng Gong
MY Ming Yu
ZH Zhi Huang
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Male NOD/SCID mice aged 5–6 weeks obtained from Wuhan Huaguenke Biotechnology Co. LTD (Hubei, China) were randomly divided into OE-NC and OE-PDIA6 groups (n = 5 for each group). This animal assay has got the approval of the Experiment Ethics Committee of Guizhou Medical University. Approximately 5 × 106 SCC9 cells with OE-NC or OE-PDIA6 stable transfection were suspended in 200 μl of PBS and then subcutaneously injected into the armpit of mice. The tumors were measured to assess tumor formation every week: tumor volume = length × width2/2. The mice were euthanized 4 weeks after injection, and the tumor xenografts were harvested and weighed.

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