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Morris water maze test

TT Tian Tian
XL Xi-run Liu
TL Ting-ting Li
ZN Zhi-chao Nie
SL Shuang-jing Li
YT Yan Tang
CG Cong-wei Gu
WX Wang-dong Xu
HJ Hong Jia
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The rats’ spatial learning and memory abilities were evaluated by the Morris water maze (MWM) test, as described previously21, before each blood draw. In the orientation-navigation test, each rat was allowed to swim and search for a platform within 120 s in 5 consecutive days. The escape latency, swimming speed and mean distance to the platform were recorded. If the rat failed to find the platform, the escape latency was recorded as 120 s. In the spatial probe test, the platform was removed on the 6th day. Each rat was allowed to swim within 120 s, and the frequency with which the rat passed the original platform quadrant was recorded. All trials were conducted between 10:00 a.m. and 13:00. The behavioural differences of the rats in each MWM test among the groups were evaluated. At week 48, the escape latencies and mean distances to the platform among groups showed a significant difference, and the rats stopped receiving food. The 48th-week indexes served as final data.

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