TIMER 2.0 Analysis

Using TIMER 2.0 (http://timer.comp-genomics.org/), we analyzed the relationship between the expression of CDCAs and infiltration levels of immune cells in liver cancer tissue. The TIMER database was used to determine the abundance of tumor infiltrates based on biomarker gene expression analysis (Li et al., 2017). Here, we chose CDCA1/3/5/8 as input and, in turn, detected cancer cells under the Immune Association module. B cells, CD8+ T cells, CD4+ T cells, neutrophils, macrophages, and dendritic cells were selected as the test types according to Li et al. (Li et al., 2016; Danaher et al., 2017). Gene expression values were transformed to Log2 RSEM values.