The method that we used in this study for behavioral assessment of focal cerebral ischemia in rats has been described previously. All rats were evaluated by the same trained person. The neurological examination results were scored according to the five point system: zero point, no obvious defect; one point, forelimb flexion; two points, forelimb flexion and lateral push resistance reduction; three points, forelimb flexion, lateral push resistance decreased, unilateral rotation occurred in three consecutive tests; four points, three points symptoms plus decreased consciousness (Yang et al., 2000).
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