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Preparation of dialysis tubing

AN Abdullah Naiyer
BK Bushra Khan
AH Afzal Hussain
AI Asimul Islam
MA Mohamed F. Alajmi
MH Md. Imtaiyaz Hassan
MS Monica Sundd
FA Faizan Ahmad
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Dialysis tubing with molecular mass cut-off (6–8 kDa) was purchased from Sigma Chemical Co. (St. Louis, USA). Pieces of dialysis tubing (4–5 inches) were prepared according to the procedure given by Dr. P. McPhie20. First, pieces of the tubing were soaked in 50% ethanol for one hour and then thoroughly washed and immersed in NaHCO3 and EDTA solution (mM ratio of 10:1) for 30–40 min at 60 °C. To prevent attack by cellulolytic microorganisms, pieces of the tubing were then washed thoroughly with distilled water and stored in 70% alcohol at 4 °C. These treatments remove all impurities such as glycerin, trace of sulfurous compounds and heavy metal ions from the cellophane tubes20. Each piece of the tubing was thoroughly washed with distilled water before filling protein solution in it.

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