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GPX activity was measured in plasma using a GPX Assay kit (Cayman 703102), according to manufacturer’s instructions. Study participants had fasted for 12 h prior to having blood drawn from the antecubital vein for both baseline and 6-month time points. Blood samples were collected in EDTA-coated tubes and centrifuged at 1000 g for 10 min (Model 614B, The Drucker Company) from which the plasma supernatant was aliquoted and stored at –80°C until analysis. Results were expressed as nmol/min/ml.
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