Antibodies and Other Reagents.

The L2 protein was detected with mouse monoclonal (mab) antibodies K4 and 33L2-1, which recognize epitopes within amino acids 20–38 and 163–170, respectively (32). The L1 protein was detected using mouse mab 33L1-7, which recognizes an epitope within amino acids 303–313 of the L1 protein (2). The TGN was detected using rabbit polyclonal antibody (pab) anti-TGN46, which that recognizes a luminal epitope within amino acid residues 200–350 (PA5-23068; Thermo Fisher Scientific). Laminin-332 (formally known as Laminin-5) was detected using rabbit pab anti–Laminin-5 (ab14509; Abcam). The nuclear envelope was detected using rabbit pab anti-LBR (ab32535; Abcam). The microtubule network was detected using mouse mab α-tubulin conjugated to AF488 (8058S; Cell Signaling). The primary antibodies were detected using goat anti-rabbit or anti-mouse AF488-conjugated secondary antibody (A-11034 and A-11029, respectively; Invitrogen). The Click-iT EdU Imaging Kits for AF555 and AF647 ({"type":"entrez-nucleotide","attrs":{"text":"C10338","term_id":"1535409"}}C10338 and {"type":"entrez-nucleotide","attrs":{"text":"C10340","term_id":"1535411","term_text":"C10340"}}C10340, respectively; Invitrogen) were used for specific detection of the EdU-labeled genome and pseudogenome. The nuclei of cells were stained with ProLong Gold and SlowFade Antifade containing DAPI ({"type":"entrez-protein","attrs":{"text":"P36931","term_id":"2506707","term_text":"P36931"}}P36931; Invitrogen). Pab rabbit anti-EEA1 (E3906; Sigma-Aldrich) was used for Western blots in the flotation assay.