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Intraperitoneal Glucose Tolerance Test
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The intraperitoneal glucose tolerance test (IPGTT) was performed 2–3 days before sacrifice. Animals were fasted overnight but allowed free access to water/infusion as appropriate. After 14–16 hours of fasting, animals were injected intraperitoneally with a 20% glucose solution at a dose of 2g/kg body weight (10µL/g). Blood glucose levels were then measured at 12 different time points (0, 3, 5, 10, 15, 20, 25, 30, 45, 60, 90 and 120 minutes).
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