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Fixed brain tissues were embedded in paraffin, and 4 μm thick coronal sections were prepared. To detect Aβ, thioflavin-S staining was performed according to the manufacturer's instructions. All slides were deparaffinized by serial hydration using a graded ethanol series, followed by treatment of the slides with 1% filtered thioflavin-S (Sigma-Aldrich) and washing. The mounted slides were stored at 4°C before fluorescence microscopy imaging (Nikon, Shinagawa, Tokyo, Japan).
Copyright and License information: ©2021 Sang Eon Park et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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