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Mice were anesthetized and the spleen was surgically removed on day 0 after intrasplenic inoculation of B16 melanoma (5 × 105) cells. Four days after the B16 inoculation, mice were injected intravenously with either RK- or GC-pulsed bone marrow-derived DCs (3 × 104 or 1 × 105). The mice were sacrificed after 2 weeks, and the liver was visually or quantitatively evaluated for B16 metastases.
Copyright and License information: ©2017 Dashtsoodol, Shigeura, Tashiro, Aihara, Chikanishi, Okada, Hanada, Sano, Kurogi and Taniguchi.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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