BMDMs or RAW264.7 cells were seeded in 12-well plates at a density of 1.5×106 cells per well and stimulated for 48 hours with medium for M0-like, LPS (50 ng/mL) and IFNγ (10 ng/mL) for M1-like, or IL4 (10 ng/mL) for M2-like polarization.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.