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BDA anterograde tracing of the corticospinal tract

XL Xiao-Hong Li
XZ Xiang Zhu
XL Xiao-Yin Liu
HX Hai-Huan Xu
WJ Wei Jiang
JW Jing-Jing Wang
FC Feng Chen
SZ Sai Zhang
RL Rui-Xin Li
XC Xu-Yi Chen
YT Yue Tu
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At 6 weeks post injury, the rats underwent anterograde tracing of the corticospinal tract with biotinylated dextran amine (BDA) (n = 4). After the rats were anesthetized, they were positioned in a stereotaxic frame. BDA (10%, 10,000 MW; Invitrogen, Eugene, OR, USA) was injected unilaterally into the left and right sensorimotor cortexes at 12 sites (0.5 μL/site) using the following coordinates (in reference to Bregma): (1) 0.5 mm anterior and 2.5 mm lateral, (2) 0.5 mm anterior and 3.5 mm lateral, (3) 1.5 mm anterior and 2.5 mm lateral, (4) 1.5 mm anterior and 3.5 mm lateral, (5) 1.0 mm posterior and 2.5 mm lateral, and (6) 1.0 mm posterior and 3.5 mm lateral. Injections were made 1.5 mm from the surface of the cortex.

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