Sequence Alignment and Phylogenetic Analysis of AhNAC TFs

PL Pengxiang Li
ZP Zhenying Peng
PX Pingli Xu
GT Guiying Tang
CM Changle Ma
JZ Jieqiong Zhu
LS Lei Shan
SW Shubo Wan
request Request a Protocol
ask Ask a question
Favorite

Arabidopsis NAC protein sequences were downloaded from the Arabidopsis Information Resource4, and sequence alignment of NAC TFs in peanut and Arabidopsis was performed by the MUSCLE method (Kumar et al., 2016). Subsequently, an unrooted phylogenetic tree was established by MEGA 7 using the neighbor-joining (NJ) method with a p-distance model and 1,000 bootstrap repeats (Kumar et al., 2018).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A