Arabidopsis NAC protein sequences were downloaded from the Arabidopsis Information Resource4, and sequence alignment of NAC TFs in peanut and Arabidopsis was performed by the MUSCLE method (Kumar et al., 2016). Subsequently, an unrooted phylogenetic tree was established by MEGA 7 using the neighbor-joining (NJ) method with a p-distance model and 1,000 bootstrap repeats (Kumar et al., 2018).
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