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Anc80L65 capsids were used to package the SpCas9 and SaCas9 systems. All the plasmids were sequenced before packaging into AAV2/Anc80L65. These viral vectors were produced by the Biolink Company (Shanghai, China). The original titers of AAV2/Anc80L65–CAG–SpCas9–WPRE–polyA, AAV2/Anc80L65–U6–(Htra2-gRNA) × 3–CAG–eGFP (Super)–bGHpolyA, and AAV2/Anc80L65–CMV–SaCas9–bGHpolyA–U6–(Htra2-gRNA) were 5.37 × 1012 viral genomes (VG)/ml, 5.51 × 1012 VG/ml, and 5.38 × 1012 VG/ml, respectively, as determined by real-time qPCR.
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