Organoid viability assay

WC Wenyi Chen
JZ Jiaqi Zhu
FL Feiyan Lin
YX Yanping Xu
BF Bing Feng
XF Xudong Feng
XS Xinyu Sheng
XS Xiaowei Shi
QP Qiaoling Pan
JY Jinfeng Yang
JY Jiong Yu
LL Lanjuan Li
HC Hongcui Cao
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Before organoid treatment with H2O2 and exosomes, or when organoids had reached the desired culture duration for analysis, expansion medium was replaced with working fluids, which contained 2 μM calcein and 8 μM propidium iodide, in accordance with the instructions of the Live & Dead Viability/Cytotoxicity Assay Kit for Animal Cells (Keygen Biotech, Nanjing, China). Organoids were observed and imaged using a confocal laser scanning microscopy (Zeiss LSM710; Carl Zeiss AG, Jena, Germany).

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