Cell Cycle Detected by Flow Cytometry

After transfection with si-NC or si-LBX2-AS1 36 h, approximately 1 × 10⁶ HCT116 cells were collected and fixed with 70% precooled ethanol for flow cytometry assay. DNA content was detected using propidium iodide (PI) (Sigma, San Antonio, USA) staining, and cell cycle distribution were detected by flow cytometry(Beckman Coulter, South Kraemer, USA), according to the methods described in a previous study.²⁵ Each experiment was repeated for 3 times independently.