Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Determination of Malondialdehyde, Glutathione, Superoxide Dismutase, and Iron Levels

GL Guangda Li
XL Xiaoxiao Li
JD Jianjian Dong
YH Yongsheng Han
request Request a Protocol
ask Ask a question
Favorite

To confirm the effect of EA on ferroptosis regulation, the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and iron were determined. Briefly, the brain tissues were isolated and perfused with 0.9% sodium chloride solution. Then, the obtained samples were mechanically homogenized, and the supernatants were collected for analysis. The levels of MDA, GSH, SOD, and total iron in the brain tissues were determined using the MDA assay kit (Cat#BC0025, Solarbio), GSH assay kit (Cat#BC1175, Solarbio), SOD assay kit (Cat#BC0175, Solarbio), and iron assay kit (Cat#A039-2-1, njjcbio6), according to the manufacturer's protocols.

Copyright and License information:  ©2021 Li, Li, Dong and Han.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A