Expression Analysis

RH Rongkui Han
MT Maria José Truco
DL Dean O. Lavelle
RM Richard W. Michelmore
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RNA-seq reads from the time course experiment described in Higashi et al. (2016) were quality and adapter trimmed using BBDuk2 and mapped to version 8 of the lettuce reference genome (Reyes-Chin-Wo et al., 2017) using STAR (Dobin et al., 2013). Oscillation of transcription level (in reads per million mapped reads) and period of oscillation were detected using R package DiscoRhythm (Carlucci et al., 2019). Transcripts with p < 0.05 over a 24-h oscillation period are reported.

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