Golgi Silver Staining

Golgi silver staining was performed as described previously (Du, 2019). The mice were sacrificed and perfused with 4% PFA. The brain was dissected, cut into half at the junction between the cortex and midbrain, and further incubated in the PFA solution for a further 10 min, followed by the immersion in the Golgi solution (FD Neurotechnologies, Rapid Golgi Kit). The Golgi solution was changed after 6 h, and the brain was kept immersed as such for 2 weeks before development as per the instructions of the manufacturer.