Caenorhabditis elegans Strains

C. elegans strain N2 (WT) was obtained from the Caenorhabditis Genetics Center (CGC). Transgenic/mutant C. elegans strains constructed or crossed by our lab were as follows (Lakso et al., 2003; Shen et al., 2020): UM0010 (P_dat–1::GFP, P_aex–3::HASN^A53T), human α-synuclein A53T overexpressing pan-neuronally (HASN^A53T OX); UM0011 (P_dat–1::GFP, P_aex–3::HASN^WT), human α-synuclein WT overexpressing pan-neuronally (HASN^WT OX); UM0012 (P_dat–1::GFP, tdp-1 KO), tdp-1 gene deletion strain; UM0013 (P_dat–1::GFP, P_aex–3::HASN^A53T, tdp-1 KO), generated from crossing of UM0010 and RB929 (tdp-1 KO); and UM0014 (P_dat–1::GFP, P_aex–3::HASN^WT, tdp-1 KO), generated from crossing of UM0011 and RB929. The transgenic strains UM0010 and UM0011 had their transgenes integrated by X-ray irradiation and were back-crossed with WT N2 4× times (Vartiainen et al., 2006b).