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2.2. Sample collection and genomic DNA extraction

KS Komal Siddiqui
AU Arsalan Ahmed Uqaili
MR Muhammad Rafiq
MB Muhammad Aqeel Bhutto
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Fresh blood (3 mL) was drawn from all the individuals in Ethylenediamine tetraacetic acid tubes. The blood was stored at 4 °C until the extraction of genome. DNA was extracted from 200 μL of whole blood by Favorgen DNA extraction kit, according to the manufacturer's instructions. Isolated DNA samples were quantified by UV spectrophotometry at 260 nm absorbance and diluted with distilled water to the concentration required for polymerase chain reaction (PCR) (100 ng/μL).

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