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EDTA anticoagulated venous blood samples were collected from median cubital vein of all participants. Peripheral blood lymphocytes were separated within subsequent 10–20 min (min) with a standard method by centrifugation over lymphocyte separation medium at 400×g for 20 min (lymphoprep™, STEMCELL technologies, Canada). The cells were washed with phosphate-buffered saline (PBS) (250×g, 10 min). Subsequently, 5 × 105/ml peripheral blood mononuclear cells (PBMCs) suspended in the PBS were used in all the experiments.
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