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Measurements of mRNA Half-Lives

JS Jae Sung Shim
SP Su-Hyun Park
DL Dong-Keun Lee
YK Youn Shic Kim
SP Soo-Chul Park
MR Mark Christian Felipe R. Redillas
JS Jun Sung Seo
JK Ju-Kon Kim
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The half-life of mRNA was determined in rice plants as previously described (Lidder et al. 2005; Park et al. 2012). Briefly, two-week-old rice seedlings were transferred soil to tap water for 2 days. After adaptation, the rice seedlings were treated with 1 mM of cordycepin (3′-deoxyadenosine) (Sigma, USA) through roots uptake for 30 min. After the treatment, the leaf tissues were harvested at indicated time points. RNA extraction, first-strand synthesis, and qRT-PCR were performed as described in “RNA Isolation and Quantitative Real-Time PCR Analysis.” Half-lives were calculated using Sigma plot software (Sigma Plot v10.0; http://www.systat.com).

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