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For PO suspension dosing (Performed at Neosome LLC)

ZA Zachary D. Aron
AM Atousa Mehrani
EH Eric D. Hoffer
KC Kristie L. Connolly
PS Pooja Srinivas
MT Matthew C. Torhan
JA John N. Alumasa
MC Mynthia Cabrera
DH Divya Hosangadi
JB Jay S. Barbor
SC Steven C. Cardinale
SK Steven M. Kwasny
LM Lucas R. Morin
MB Michelle M. Butler
TO Timothy J. Opperman
TB Terry L. Bowlin
AJ Ann Jerse
SS Scott M. Stagg
CD Christine M. Dunham
KK Kenneth C. Keiler
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Female CD-1 mice as above were fasted for 2 h prior, and 4 h after dosing. MBX-4132 was administered at 10 mL/kg via oral gavage with a 1.0, 2.5, or 10.0 mg/ml suspension in vehicle A (5% DMSO, 5% Cremophor EL®, 0.45% hydroxypropylmethylcellulose, 0.45% alginic acid, 22.5% hydroxy-betacyclodextrin). At 0.25, 0.5, 1, 2, 4, 8, and 24 h post dose, 3 mice from each group were euthanized by CO2 inhalation, blood was collected by cardiac puncture into K2EDTA collection tubes, and protein was precipitated and analysed by LC/MS-MS for plasma concentrations of MBX-4132. Data were analysed using WinNonLin.

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