Synovial fluid and synovial fluid mononuclear cell isolation

JJ Ji Hye Jeong
SC Su Jin Choi
SA Soo Min Ahn
JO Ji Seon Oh
YK Yong-Gil Kim
CL Chang-Keun Lee
BY Bin Yoo
SH Seokchan Hong
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Aspirated synovial fluid samples were immediately transferred to the laboratory and synovial fluid mononuclear cells (SFMCs) were isolated using Ficoll-Paque™ PLUS gradient centrifugation (GE Healthcare, Piscataway, NJ). Supernatants were stored at − 80 °C until the measurement of MPO-dsDNA complexes.

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