Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Genetic material and generation of an F1 hybrid population

DK Dominic Knoch
CW Christian R. Werner
RM Rhonda C. Meyer
DR David Riewe
AA Amine Abbadi
SL Sophie Lücke
RS Rod J. Snowdon
TA Thomas Altmann
ask Ask a question
Favorite

The experimental materials consisted of 477 spring-type B. napus (canola) genotypes with double-low seed quality (low erucic acid and low glucosinolate content) from a previously described breeding programme (Jan et al. 2016, 2019; Knoch et al. 2020). The largest proportion, 475 lines, comprised genetically diverse pollinator lines that could be assigned to three breeding pools (referred to as breeding pools 1, 2 and 3). The pollinator lines were derived from 184 crosses, with some elite parents used in several crosses. The F1 hybrid population with 950 individuals was created by crossing the 475 pollinators with two elite male-sterile testers (MS1 and MS2) from a pool of testers carrying the Male Sterility Lembke (MSL) sterility system (NPZ Lembke, Hohenlieth, Germany).

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A