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Examination of miRNA

QZ Qian Zhang
XS Xin Song
PM Ping Ma
LL Liping Lv
YZ Yangyang Zhang
JD Jiang Deng
YZ Yanyu Zhang
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miRNA expression was determined with a modified protocol from our previous report (15). Briefly, cDNA synthesis such that a poly(A) tail followed each miRNA was performed by reverse transcription (RT) using a tagged poly(T) primer (5′-CAGGTCCAGTTTTTTTTTTTTTTTVN-3′) and the One-Step miRNA cDNA Synthesis Kit (Hai-gene Bio Inc., Harbin, China). The generated cDNA was then used for miRNA examination (primers listed in Supplementary 5). The relative expression level of each miRNA was normalized to the expression of U6, and the cycle threshold (CT) ranged from 15 to 17 cycles.

Copyright and License information:  ©2021 Zhang, Song, Ma, Lv, Zhang, Deng and Zhang.
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