2.2.4. Hydroalcoholic Extracts

The extracts were obtained by following the protocol reported by Etame Loé et al. [15]. For this purpose, 100 g powder was added to 300 mL of palm wine and boiled at 100°C for 15 min. After 6 h in the dark at room temperature, the mixture was filtered using Whatman No. 1 filter paper. The filtrate was dried in an oven at 45°C to obtain a crude hydroalcoholic extract.

From each plant material and extraction solvent, the extract was weighed and the yield (%) calculated based on the mass of the sample of plant material. Those extracts were frozen during antibacterial evaluation.