Mouse urine samples were collected for 24 h once every two weeks using metabolic cage (AS ONE Corporation, Osaka, Japan). Urinary protein and creatinine were measured by Bradford method (Bio-Rad, CA, USA) and Jaffe’s method (Wako Pure Chemicals, Osaka, Japan), respectively, as described previously [14]. Urinary protein concentration was normalized with urinary creatinine concentration, and presented as proteinuria score.
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