Transmission electron microscopy of bacterial cells

MT Man Tang
YZ Yanchao Zhou
JG Jiayang Gao
JP Jingli Peng
YW Yuan Wang
QZ Qirui Zhao
LL Lihao Liao
KW Kai Wang
MP Mengjia Pan
MX Meng Xing
WP Wen Pan
DD Danling Dai
MF Min Fu
LY Li Yu
CZ Chuqing Zhang
YW Yuchuan Wang
YZ Ying Zhang
LX Li Xu
JL Jing Li
XB Xiao Bao
WP Wenxian Piao
SL Shihong Lin
KL Kaibei Lu
XZ Xuelan Zhang
WC Weiguo Cao
KY Kai Yang
ZH Zhumei He
SW Shaoping Weng
QL Qiuyun Liu
JH Jianguo He
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Samples containing bacterial cells in PBS were incubated with conjugates at 30 °C (R. solanacearum) or 37 °C (rest) at concentration of 1 mg/ml. Controls containing bacterial cells in PBS were incubated without conjugates at the same temperature. Subsequently, all samples were centrifuged at 7,300× g for 1 min, and washed with PBS twice followed by centrifugation. A drop containing the bacteria was absorbed onto a copper grid and negatively stained with phosphotungstic acid for 30 s. The grids were examined using a JEM-100CX-II electron microscope (JEOL Ltd., Japan)17.

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