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Antioxidant activity determination by DPPH radical scavenging assay

JD Jason Darmadi
RB Razethy Rahayu Batubara
SH Sandiego Himawan
NA Norma Nur Azizah
HA Hilyatushalihah Kholis Audah
AA Ade Arsianti
EK Evi Kurniawaty
II Intan Safinar Ismail
IB Irmanida Batubara
KA Kholis Abdurachim Audah
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Antioxidant assay was performed as reported by Jadid et al.15, with several modifications in the procedure. Ethyl acetate extract and ascorbic acid (Merck, Germany) dissolved in 75% ethanol at concentrations 0–100 ppm were mixed with DPPH (Sisco Research Laboratories, India) 100 ppm (1:1). The mixtures were incubated for 30 min in a dark chamber at 25 °C and read at 517 nm UV–vis with both DPPH IC50 made from standard control calibration curve DPPH at concentration 0–100 ppm. Both IC50 were then compared with each other.

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