Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
E. coli fitness (survival) assay
Request a Protocol
Ask a
question
Favorite
Overnight cultures were inoculated from frozen stocks. 100 μl of each strain was spread on each plate in triplicate and allowed to dry by evaporation. At each time point, the number of viable cells was determined by “coring” a section of the lawn with a sterile Pasteur pipet (inner diameter ~ 1.5 mm), resuspending each core in 50 μl of LB, and titering by serial dilution and plating onto LB medium; the limit of detection is < 100 cfu/ml54,55.
Copyright and License information: The Author(s) ©2021
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
