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Calibration standards and quality control (QC) samples
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The primary stock solutions of GCK (0.408 mg/mL), PPD (0.402 mg/mL) and PPT (IS, 0.196 mg/mL) were prepared by dissolving each compound in methanol, respectively. Working solutions were prepared by diluting the stock solutions appropriately with methanol-water (V:V = 1:1) and stored at 4 ℃. The standard calibration curves samples and QC samples were prepared by spiking 2.5 µL of working solution with 100 µL of gut microbiota work solution and 398 µL of GAM broth. The solution of PPT (IS) was diluted to 1.96 µg/mL using methanol-water.
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