Decolourisation of Reactive Red 120 was determined by colourimetric determination. The absorbance of each solution was determined using a 525 nm wavelength against blank using a Shimadzu U.V. Mini 1240 spectrophotometer. Meanwhile, bacterial growth was measured using a colony-forming unit per millilitre technique (CFU/mL) and optical density (OD) at 600 nm [21].
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