Lipoxygenase Enzyme (LOX) Inhibition Assay

LOX Inhibition was determined by applying the previously reported method.¹⁴ Briefly, sodium phosphate buffer 0.1M (pH 8.0) and soybean lipoxygenase (165 U/mL) and 10 µL of different concentrations of REVERC3 and regular turmeric extract were combined and incubated at 25°C for 10 min. 10 μL of 0.32 mM substrate in the form of sodium linoleic acid solution was added to initiate the reaction. The formation of (9Z, 11E)- (13S)-13-hydroperoxyoctadeca-9,11-dienoate from sodium linoleic acid by enzymatic conversion was determined by measuring the change in absorbance at 234 nm using UV-Vis spectrophotometer. Negative control was drawn up by replacing samples with sodium phosphate buffer and DMSO into the quartz cuvette. All the reactions were carried out in triplicates.