Transmission Electron Microscopy (TEM) of the Bacteriophage p54

FK Fazal Mehmood Khan
VG Vijay Singh Gondil
CL Changchang Li
MJ Mengwei Jiang
JL Junhua Li
JY Junping Yu
HW Hongping Wei
HY Hang Yang
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The bacteriophage p54 was purified and concentrated using cesium chloride density gradient ultracentrifugation on 35,000 rpm for 2 h at 4°C. The concentrated bacteriophage was stained with freshly prepared phosphotungstic acid solution (2%) for 3 min and spot dried on the copper grid for 2 h. The samples were examined on H-7000FA transmission electron microscope (Hitachi, Japan).

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