The bacteriophage p54 was purified and concentrated using cesium chloride density gradient ultracentrifugation on 35,000 rpm for 2 h at 4°C. The concentrated bacteriophage was stained with freshly prepared phosphotungstic acid solution (2%) for 3 min and spot dried on the copper grid for 2 h. The samples were examined on H-7000FA transmission electron microscope (Hitachi, Japan).
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