Thoracotomy was performed on a randomly selected portion of SCI rats at the 60 days after cell transplantation. The heart was infused with 500 ml paraformaldehyde buffer solution, and the spinal cord was carefully separated. The spinal cord was placed in 2% paraformaldehyde for 6 h. H&E staining was performed and observed under an optical microscope. For nissl staining, the frozen sections were immersed in chloroform for 1 min and gradient alcohol for 1 min; respectively. After being rinsed twice with distilled water, they were dyed in tar purple dye for 30 min, and then dyed in a 37 °C box for 10 min. After being rinsed twice again by distilled water, 95% alcohol was used, followed by conventional dehydration, transparency, and tablet sealing treatment. Images were observed and photographed under an optical microscope.
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