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To compare the apoptotic cell numbers of each group, zebrafish larvae at 4 dpf were incubated in 10 μg/ml of AO (Thermo Fisher, A1301, United States) in the culture medium. Phenylthiourea (PTU) treatment can be applied to reduce pigmentation after 24 hpf. After 30 min of staining, larvae were washed three times in culture medium. Larvae were then transferred to 48-well plates and anesthetized with tricaine for imaging (Corning, catalog #4520, Corning, NY). Images were taken in GFP channels (n = 25 per group).
Copyright and License information: ©2021 Han, Zhang, Dong, Wang and Wang.
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