2.3.2. Spore Germination Inhibition Assay

The spore germination inhibition was assessed following the method described by Bammou et al. [16]. Briefly, an aliquot (100 µL) of M. scaettae spore suspension adjusted to 10³ spores/mL of sterile distilled water (by means of a Malassez hemocytometer) was spread over the Petri dishes containing the PDA medium supplemented with plant extracts as indicated above. The number of spores germinating out of 100 counted was determined after 24 hours of incubation at 25 ± 2°C. A spore was considered as germinating when the length of its germ tube was greater than its smallest diameter. Each treatment included four replicates and a negative control containing unsupplemented media. The percentage of spore germination inhibition (PSGI) by each plant extract was calculated using the following equation:

where N_c: number of germinating spores in the control and N_t: number of germinating spores in the presence of the plant extract.