2.3. Scale-Up of TSNP Production

Scaling up the TSNP synthesis was achieved by increasing all the reactants’ volumes from the adapted method proportionally to reach a final volume of 200 mL. An Ag seed volume of 20 mL was prepared by mixing 8.53 mL DW, 0.95 mL TSC (25 mM), 0.47 mL PSSS (500 mg/mL), and 0.57 mL NaHB₄ (10 mM). A volume of 9.48 mL of AgNO₃ (0.5 mM) was added at a rate of 3.79 mL/min. After 4 hours, the preparation of the seeds was complete and 8.75 mL of seed solution (25.56 ppm) was mixed with 100 mL of DW and 1.87 mL of AA (10 mM). 75 mL of AgNO₃ (0.5 mM) was added at a rate of 25 mL/min, followed by 12.5 mL of TSC (25 mM) after the reaction was finished.