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4.8. Total RNA and miRNA Extraction

SY Swee Keong Yeap
NA Norlaily Mohd Ali
MA Muhammad Nadeem Akhtar
NR Nursyamirah Abd Razak
ZC Zhi Xiong Chong
WH Wan Yong Ho
LB Lily Boo
SZ Seema Zareen
TK Tonni Agustiono Kurniawan
RA Ram Avtar
SN Stephanie Y. L. Ng
AO Alan Han Kiat Ong
NA Noorjahan Banu Alitheen
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Total RNAs including small RNAs were isolated from MCF-7 treated with BHMC using miRNeasy kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions. The quality of extracted RNA was assessed by the NanoDrop-1000 Spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and Agilent 2100 Bioanalyzer, and samples with A260/A280 ratio between 1.8 to 2.1 and an RIN value of 8 and above was used for further analysis.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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