RNA extraction and cDNA synthesis

Total RNA was extracted from all TF samples using the RNeasy Plus Mini Kit (Qiagen, HildenGermany). Five TR samples were also analyzed as standard control isolates. The RNA quality and quantity were analyzed using 1% agarose gel electrophoresis and spectrophotometer (Eppendorf BioPhotometer Plus, Eppendorf, Germany), respectively. Then, cDNA was synthesized using high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA) with oligo dT and random hexamer primers based on the manufacturer’s instruction.