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Chondrocyte apoptosis was examined using an Annexin V-FITC apoptosis detection kit. Chondrocytes were cultured in 6-well plates at a density of 100,000 cells per well. After the respective treatments, the chondrocytes were digested, centrifuged and resuspended in 100 μL of binding buffer. The chondrocytes were then incubated with 5 μL of Annexin V-FITC and 10 μL of propidium iodine (PI) at room temperature for 15 minutes. The chondrocyte apoptotic rate was measured using a flow cytometer (Accuri C6, BD Biosciences, San Jose, CA, USA).
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