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Demultiplexing was performed using bcl2fastq (v.2.17.1.14), allowing 1 mismatch when assigning reads to barcodes. Adapters (Read1:AGATCGGAAGAGCACACGTCTGAACTCCAGTCA, Read2:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT) were trimmed during the demultiplexing process. After trimming and demultiplexing, two compressed FASTQ files (“fastq.gz”) for each sample were obtained.
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