Telomeric repeat amplification protocol (TRAP) assay

RB Ryan M. Baxley
WL Wendy Leung
MS Megan M. Schmit
JM Jacob Peter Matson
LY Lulu Yin
MO Marissa K. Oram
LW Liangjun Wang
JT John Taylor
JH Jack Hedberg
CR Colette B. Rogers
AH Adam J. Harvey
DB Debashree Basu
JT Jenny C. Taylor
AP Alistair T. Pagnamenta
HD Helene Dreau
JC Jude Craft
EO Elizabeth Ormondroyd
HW Hugh Watkins
EH Eric A. Hendrickson
EM Emily M. Mace
JO Jordan S. Orange
HA Hideki Aihara
GS Grant S. Stewart
EB Edward Blair
JC Jeanette Gowen Cook
AB Anja-Katrin Bielinsky
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TRAP assays were performed following the manufacturer’s protocol for the TRAPeze Telomerase Detection Kit (EMD-Millipore S7700). Briefly, whole cell extracts were prepared using 1x CHAPS lysis buffer and stored at −80 °C. Protein concentration was measured using Protein Assay Dye (BioRad #500-0006) in comparison to a BSA (NEB B9000S) standard curve following standard protocols. TRAP reactions utilized Platinum Taq DNA Polymerase (Invitrogen 10966) and were resolved on 10% polyacrylamide 0.5x TBE gels, stained for 1 h with SYBR GOLD (Invitrogen S11494) and detected with a Typhoon FLA 9500 imager and processed using FIJI version 2.1.0/153.c and Adobe Photoshop version 21.2.1.

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