TRAP assays were performed following the manufacturer’s protocol for the TRAPeze Telomerase Detection Kit (EMD-Millipore S7700). Briefly, whole cell extracts were prepared using 1x CHAPS lysis buffer and stored at −80 °C. Protein concentration was measured using Protein Assay Dye (BioRad #500-0006) in comparison to a BSA (NEB B9000S) standard curve following standard protocols. TRAP reactions utilized Platinum Taq DNA Polymerase (Invitrogen 10966) and were resolved on 10% polyacrylamide 0.5x TBE gels, stained for 1 h with SYBR GOLD (Invitrogen S11494) and detected with a Typhoon FLA 9500 imager and processed using FIJI version 2.1.0/153.c and Adobe Photoshop version 21.2.1.
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