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Gene cloning

YH Yangjie Hu
MO Moutasem Omary
YH Yun Hu
OD Ohad Doron
LH Lukas Hoermayer
QC Qingguo Chen
OM Or Megides
OC Ori Chekli
ZD Zhaojun Ding
JF Jiří Friml
YZ Yunde Zhao
IT Ilan Tsarfaty
ES Eilon Shani
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Cell-type-specific YUC1 and TAA1 inducible lines were generated by cloning the YUC1-2A-TAA1 cassette into XhoI and SpeI sites of the pER8 vector59. The full-length cDNA of YUC1 was cloned into the BamHI site, and the full-length cDNA of TAA1 was cloned into the BglII site of the pM2A vector containing 2 A peptides60 to generate the complete YUC1-2A-TAA1 cassette. For construction of the YUC1-2A-TAA1-2A-GFP cassette, GFP was cloned into the SmaI site of the pM2A vector containing YUC1-2A-TAA1. For the tissue-specific activation of the YUC1-2A-TAA1 or the YUC1-2A-TAA1-2A-GFP cassette, the genomic DNA containing promoters of SHR, SCR, APL, CLE40, WOX5, or WER were used. The primers are listed in Supplementary Table 2. The floral dipping method was used in transformation of Arabidopsis. At least ten independent lines were generated, and two of each transformation were obtained and analyzed in T3 homozygous lines.

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