GMP NK Cell Expansion With uAPC

NK cells were expanded using the G-Rex® bioreactor (Saint Paul, Minnesota, USA) in co-cultures with uAPC for the CB-NK CAR clinical trial (21) (NCT Number: {"type":"clinical-trial","attrs":{"text":"NCT03056339","term_id":"NCT03056339"}}NCT03056339). This closed cell culture system enables using flasks with a gas-permeable membrane, allowing for optimal gaseous exchange and ensuring aerobic growth kinetics. Using previously optimized variables which included cell seeding density, calibrated media volume, and media formulation, we were able to adapt the G-Rex M100 series from pre-clinical protocols to translate and linearly scale the procedure to the desired yield in the GMP setting.